Dionex戴安-色谱柱Acclaim PA2, 3 µm Analytical, （4.

Acclaim Polar Advantage II (PA2) columns feature a patented bonding chemistry that provides enhanced hydrolytic stability from pH 1.5–10 and allows resolution of a wide variety of polar and nonpolar analytes. The Acclaim PA2 is compatible with 100% aqueous phases and exhibits high reversed-phase capacity, with selectivity complementary to conventional C18 columns such as the Acclaim 120 C18. As with all Acclaim columns, the Acclaim PA2 is LC/MS-compatible. • Single-run analysis of polar and nonpolar analytes • Hydrolytically stable (pH 1.5–10) • Rugged column with high efficiency, symmetrical peak shapes even with basic and acidic compounds • Compatible with 100% aqueous phases • High reversed-phase capacity with selectivity complementary to conventional C18 columns Acclaim PA2 columns are compatible with 100% aqueous phases, overcoming many of the limitations of conventional C8 and C18 reversed-phase columns. Conventional reversedphase columns are unstable under these conditions and rapidly lose their ability to retain analytes. Acclaim PA2 columns are available both in the 3- and 5-μm particle sizes and 4.6-, 3.0, and 2.1 mm- diameters, with and average pore diameter of 120Å. Acclaim PA2 may be used for some LC/MS applications. These columns are available for fast LC; refer to the RSLC section. Acclaim PA2: A Polar-Embedded Reversed-Phase Column with Selectivity Complementary to Conventional C18 Columns Specifications pH range 1.5–10 End-capped Yes Metal impurity (Na, Fe, Al) <10.0 ppm Total Metal <3.0 ppm Individual Metal Pore volume 0.9–1.0 mL/g Average pore diameter 120 Å Surface area 300 m2/g Temperature < 60 oC Starting material Ultrapure silica Particle size 3 and 5 µm Particle shape Spherical Particle size distribution (40/90) <1.3 Total carbon content 17% Carbon chain length C18